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phospho pi3k p85 alpha tyr607  (Proteintech)


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    Proteintech phospho pi3k p85 alpha tyr607
    Fig. 1. Insulin resistance and lipid deposition were observed in the liver of db/db mice. (a) Representative western blot maps of IR protein and original blots are presented in Supplementary File 2 Fig. 1. (b) Protein level of IRβ. (c) Protein level of <t>PI3K.</t> (d) Protein level of <t>P-PI3K.</t> (e) Expression level of p-PI3K/PI3K. (f) Protein level of AKT. (g) Protein level of p-AKT. (h) Expression level of p-AKT/AKT. (i) Representative images of liver tissues stained with hematoxylin and eosin (H&E) and Oil Red O (scale bars = 50 μm) are shown. (j) Fat drop area (%). (k) FFA content in the liver. (l) Hepatic levels of TGs. (m) Liver index. Data are mean ± SEM. n = 3 (2 images analyzed per sample for Oil Red O-stained images). n = 8 per group (western blotting, liver FFA, TG, and liver index analyses). Student’s t-test was performed. *P < 0.05, **P < 0.01.
    Phospho Pi3k P85 Alpha Tyr607, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho pi3k p85 alpha tyr607/product/Proteintech
    Average 96 stars, based on 256 article reviews
    phospho pi3k p85 alpha tyr607 - by Bioz Stars, 2026-03
    96/100 stars

    Images

    1) Product Images from "Characteristics of different lipid droplet-mitochondrial contacts patterns during lipid droplet metabolism in T2DM-induced MASLD."

    Article Title: Characteristics of different lipid droplet-mitochondrial contacts patterns during lipid droplet metabolism in T2DM-induced MASLD.

    Journal: Scientific reports

    doi: 10.1038/s41598-025-87871-2

    Fig. 1. Insulin resistance and lipid deposition were observed in the liver of db/db mice. (a) Representative western blot maps of IR protein and original blots are presented in Supplementary File 2 Fig. 1. (b) Protein level of IRβ. (c) Protein level of PI3K. (d) Protein level of P-PI3K. (e) Expression level of p-PI3K/PI3K. (f) Protein level of AKT. (g) Protein level of p-AKT. (h) Expression level of p-AKT/AKT. (i) Representative images of liver tissues stained with hematoxylin and eosin (H&E) and Oil Red O (scale bars = 50 μm) are shown. (j) Fat drop area (%). (k) FFA content in the liver. (l) Hepatic levels of TGs. (m) Liver index. Data are mean ± SEM. n = 3 (2 images analyzed per sample for Oil Red O-stained images). n = 8 per group (western blotting, liver FFA, TG, and liver index analyses). Student’s t-test was performed. *P < 0.05, **P < 0.01.
    Figure Legend Snippet: Fig. 1. Insulin resistance and lipid deposition were observed in the liver of db/db mice. (a) Representative western blot maps of IR protein and original blots are presented in Supplementary File 2 Fig. 1. (b) Protein level of IRβ. (c) Protein level of PI3K. (d) Protein level of P-PI3K. (e) Expression level of p-PI3K/PI3K. (f) Protein level of AKT. (g) Protein level of p-AKT. (h) Expression level of p-AKT/AKT. (i) Representative images of liver tissues stained with hematoxylin and eosin (H&E) and Oil Red O (scale bars = 50 μm) are shown. (j) Fat drop area (%). (k) FFA content in the liver. (l) Hepatic levels of TGs. (m) Liver index. Data are mean ± SEM. n = 3 (2 images analyzed per sample for Oil Red O-stained images). n = 8 per group (western blotting, liver FFA, TG, and liver index analyses). Student’s t-test was performed. *P < 0.05, **P < 0.01.

    Techniques Used: Western Blot, Expressing, Staining



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    Fig. 1. Insulin resistance and lipid deposition were observed in the liver of db/db mice. (a) Representative western blot maps of IR protein and original blots are presented in Supplementary File 2 Fig. 1. (b) Protein level of IRβ. (c) Protein level of <t>PI3K.</t> (d) Protein level of <t>P-PI3K.</t> (e) Expression level of p-PI3K/PI3K. (f) Protein level of AKT. (g) Protein level of p-AKT. (h) Expression level of p-AKT/AKT. (i) Representative images of liver tissues stained with hematoxylin and eosin (H&E) and Oil Red O (scale bars = 50 μm) are shown. (j) Fat drop area (%). (k) FFA content in the liver. (l) Hepatic levels of TGs. (m) Liver index. Data are mean ± SEM. n = 3 (2 images analyzed per sample for Oil Red O-stained images). n = 8 per group (western blotting, liver FFA, TG, and liver index analyses). Student’s t-test was performed. *P < 0.05, **P < 0.01.
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    Fig. 1. Insulin resistance and lipid deposition were observed in the liver of db/db mice. (a) Representative western blot maps of IR protein and original blots are presented in Supplementary File 2 Fig. 1. (b) Protein level of IRβ. (c) Protein level of <t>PI3K.</t> (d) Protein level of <t>P-PI3K.</t> (e) Expression level of p-PI3K/PI3K. (f) Protein level of AKT. (g) Protein level of p-AKT. (h) Expression level of p-AKT/AKT. (i) Representative images of liver tissues stained with hematoxylin and eosin (H&E) and Oil Red O (scale bars = 50 μm) are shown. (j) Fat drop area (%). (k) FFA content in the liver. (l) Hepatic levels of TGs. (m) Liver index. Data are mean ± SEM. n = 3 (2 images analyzed per sample for Oil Red O-stained images). n = 8 per group (western blotting, liver FFA, TG, and liver index analyses). Student’s t-test was performed. *P < 0.05, **P < 0.01.
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    Image Search Results


    Fig. 1. Insulin resistance and lipid deposition were observed in the liver of db/db mice. (a) Representative western blot maps of IR protein and original blots are presented in Supplementary File 2 Fig. 1. (b) Protein level of IRβ. (c) Protein level of PI3K. (d) Protein level of P-PI3K. (e) Expression level of p-PI3K/PI3K. (f) Protein level of AKT. (g) Protein level of p-AKT. (h) Expression level of p-AKT/AKT. (i) Representative images of liver tissues stained with hematoxylin and eosin (H&E) and Oil Red O (scale bars = 50 μm) are shown. (j) Fat drop area (%). (k) FFA content in the liver. (l) Hepatic levels of TGs. (m) Liver index. Data are mean ± SEM. n = 3 (2 images analyzed per sample for Oil Red O-stained images). n = 8 per group (western blotting, liver FFA, TG, and liver index analyses). Student’s t-test was performed. *P < 0.05, **P < 0.01.

    Journal: Scientific reports

    Article Title: Characteristics of different lipid droplet-mitochondrial contacts patterns during lipid droplet metabolism in T2DM-induced MASLD.

    doi: 10.1038/s41598-025-87871-2

    Figure Lengend Snippet: Fig. 1. Insulin resistance and lipid deposition were observed in the liver of db/db mice. (a) Representative western blot maps of IR protein and original blots are presented in Supplementary File 2 Fig. 1. (b) Protein level of IRβ. (c) Protein level of PI3K. (d) Protein level of P-PI3K. (e) Expression level of p-PI3K/PI3K. (f) Protein level of AKT. (g) Protein level of p-AKT. (h) Expression level of p-AKT/AKT. (i) Representative images of liver tissues stained with hematoxylin and eosin (H&E) and Oil Red O (scale bars = 50 μm) are shown. (j) Fat drop area (%). (k) FFA content in the liver. (l) Hepatic levels of TGs. (m) Liver index. Data are mean ± SEM. n = 3 (2 images analyzed per sample for Oil Red O-stained images). n = 8 per group (western blotting, liver FFA, TG, and liver index analyses). Student’s t-test was performed. *P < 0.05, **P < 0.01.

    Article Snippet: The membrane was then washed three times with TBST and incubated with HRP-labeled secondary antibodies at room temperature for 1 h. Details of primary and secondary antibodies used in this study are as follows: Insulin Receptor β (4B8) Rabbit mAb(1:1000, 3025 S, Cell Signaling); PI3 Kinase p85 Alpha Monoclonal antibody(1:20000, 60225-1-Ig, Proteintech); Phospho-PI3K p85 alpha (Tyr607) Antibody(1:1000, #AF3241, Affinity); Akt (pan) (11E7) Rabbit mAb (1:1000, 4685 S, Cell Signaling); Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (1:1000, 4060 S, Cell Signaling); GAPDH Polyclonal antibody(1:20000, 10494-1-AP, Proteintech); VDAC1/2 Polyclonal antibody(1:2000, 10866-1-AP, Proteintech); ATGL Antibody(1:2000, 2138 S, Cell Signaling); ADRP/Perilipin-2 Polyclonal antibody(1:10000, 15294-1- AP, Proteintech); Anti-Mitofusin 2 antibody [NIAR164] (1:5000, ab124773, Abcam); DRP1 Antibody (C-5) (1:1000, sc-271583, SANTA); COX4I1 Monoclonal Antibody(1:2000, E-AB-22002, Cell Signaling); CPT1A Polyclonal antibody(1:1000, 15184-1-AP, Proteintech); Acetyl-CoA Carboxylase (C83B10) Rabbit mAb(1:1000, 3676 S, Cell Signaling); Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb(1:1000, 11818 S, Cell Signaling); Anti-mouse IgG, HRP-linked Antibody(1:2000, 7076P2, Cell Signaling); Anti-rabbit IgG, HRPlinked Antibody(1:2000, 7074P2, Cell Signaling).

    Techniques: Western Blot, Expressing, Staining